axt file format and negative strands
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9.6 years ago
ahmad • 0

Hello

I'm using the axt alignments format between a primary organism and an aligning organism. I'm a bit confused about the - strand definition which is the following:

If the strand value is "-", the values of the aligning organism's start and end fields are relative to the reverse-complemented coordinates of its chromosome.

For example if I have the following:

33 chr1 48732 48904 chr1 151609259 151609413 - 8362

GTCAGCTCTACTCTGACTTCGCTAGAGGCTCCAGTGCT-GTCCCTGCAGTAAAGGACCTCATGGTTCTAAAACCCCAGGTCTTCCTTATCCTGGAGGGCATGTTGTGTTCATATAAAAGAGCATCACGATTATGTTTAATACTTCtagttgtcccagtgtttacagtgtacgct

GCCAGCTCTACTCTGACTTCCCAGGAGACTCCAGTGCTTGTCCCTGTAGTAAGGGACCACACGATTCTAAAGTTCCAGCTCTTTCCTAATCTGCAGGACATGTTTT------------------CACAATTATttttaatgcttgtagt-gtcctggtgtttacagtgtatact

The aligning organism's start and end fields are 151609259 and 151609413 on the - strand. How can I get the actual coordinates of the aligning organism based on this? any help would be greatly appreciated..

next-gen • 1.7k views
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