Entering edit mode
9.6 years ago
ahmad
•
0
Hello
I'm using the axt alignments format between a primary organism and an aligning organism. I'm a bit confused about the - strand definition which is the following:
If the strand value is "-", the values of the aligning organism's start and end fields are relative to the reverse-complemented coordinates of its chromosome.
For example if I have the following:
33 chr1 48732 48904 chr1 151609259 151609413 - 8362
GTCAGCTCTACTCTGACTTCGCTAGAGGCTCCAGTGCT-GTCCCTGCAGTAAAGGACCTCATGGTTCTAAAACCCCAGGTCTTCCTTATCCTGGAGGGCATGTTGTGTTCATATAAAAGAGCATCACGATTATGTTTAATACTTCtagttgtcccagtgtttacagtgtacgct
GCCAGCTCTACTCTGACTTCCCAGGAGACTCCAGTGCTTGTCCCTGTAGTAAGGGACCACACGATTCTAAAGTTCCAGCTCTTTCCTAATCTGCAGGACATGTTTT------------------CACAATTATttttaatgcttgtagt-gtcctggtgtttacagtgtatact
The aligning organism's start and end fields are 151609259 and 151609413 on the - strand. How can I get the actual coordinates of the aligning organism based on this? any help would be greatly appreciated..