Entering edit mode
9.6 years ago
Michel Edwar
▴
80
I have done a phylogeny tree using protein sequences. Now I want to measure dn/ds on several branches. If I want to work on the nucleotide level, can I do the analysis using the corresponding coding region only or do I need to include the introns as well?
Building on this, you can't just give it -any- coding sequence. It needs to be in the appropriate frame or the analysis is meaningless.