What kind of information is good to know about a protein (or domain) and where can we find it?
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9.4 years ago
mparadis92 ▴ 40

Hello. This is kind of a weird question and I'm going to try to make it easier to understand. I'm a student in bioinformatics for only 2 years now so go easy on me :)

Long story short, I predicted disorder scores for every amino acids in every protein from ~50 phages (about 3000 proteins) using DISOPRED3. Only a few had domains of about 30 disordered residues. I want to get the most information about the protein closest to the consensus of an alignment I did with all those highly disordered proteins.

I'm a good programmer but I lack skills and knowledge when it's time to "talk" about a protein or a domain. I know I can use PDB or other databases but why? To find a protein with a similar domain and get information about it's function? What is the typical process to gather useful information about proteins?

Thanks a lot to whoever takes the time to help me understand the way to do this the right way!

protein PDB domain DISOPRED3 • 2.2k views
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It depends on what you want to know about the protein which in turn depends on the question you're interested in. What do you want to know/achieve by predicting disordered domains ? What's the question/hypothesis behind your work ?

This being said, information about proteins is scattered in different databases with each usually focusing on a particular type on information. However a good starting point would be Uniprot which organizes and centralizes all types of protein annotation.

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9.4 years ago
candyton99 • 0

Usually, we'd use a tool to help analyse the protein. And I think that's what most researchers do while trying to gather information, PRECIS, for instance.

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