Documentation On Solexa Sequencing Process

Question

[Steffi]

Can one point out some useful and detailed reference on the illumina sequencing protocol (single end and paired-end reads)? So - readable also for not-biologists but that you still know what is going on?

I find it especially hard to find some good documentation on the paired-end reads workflow.

Thanks a lot, steffi

[Damian Kao]

You mean the sequencing chemistry or the actual wet lab protocols?

[Steffi]

I would be interested in both. So, on the one hand, the individual steps of the library preparation and, as well, how the sequencing process is working.

Answer 1

[User 59]

Forget reading, watch instead!

http://www.youtube.com/watch?v=77r5p8IBwJk

[Steffi]

thanks, I've already seen this video... :) I would like to have some more details. So e.g. which adaptor is supposed to be part of the read. How do things change when looking at paired-end reads?