how to work with sff file generate from roche_454

Question

[debashis.bioinfo]

Hello all,

I want to do the genome assembly. Now I have the sff file generated from roche-454. First, I want to do the quality filteration process and after that I want do the assembly. Please let me know which software will be the best one for low quality base trimming and assembly of the reads.

Thanking You,

With Regards,
Debashis

Answer 1

[rtliu]

Some sff background information - here

Newbler is the best software for 454 data. You may request one from here