How to find out off target reads?
0
0
Entering edit mode
9.4 years ago

Here is the summary of Ion Torrent run result. Valid reads is only 19% how to improve it? How to find reads outside target region?

Reads                   21481392
Mapped Reads            19442218
Mapped %                90.50725391
Valid Reads             19.67%
Total-Reads on Target   3823517
On Target               24.72%
Off Target Reads        15618701
iontorrent RNA-Seq Off-Target-Reads • 2.8k views
ADD COMMENT
3
Entering edit mode

Can't resist citing @BioMickWatson ...

ADD REPLY
0
Entering edit mode

what the meaning of ur message?

ADD REPLY
0
Entering edit mode

Oh, come on... I agree for Solid, but surely Ion Torrent is much better.

ADD REPLY

Login before adding your answer.

Traffic: 1963 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6