Entering edit mode
9.4 years ago
Mingkun
▴
40
I am trying to generate blastn output to be used in MEGAN, the command line I used is
blastall -p blastn -d nt -a 8 -F T -b 10 -v 10 -e 1e-5 -m 8 -i III -o RRR.blastn
I sometimes got more than 600 HSP for one query, although the total number of hit-genome was 10, the HSP per hit-genome could more than 10, is there a way to set number of HSP per hit-genome? I have tried the -K
, -B
, and --max_target_seqs
in new blastn, none of them helps. Anyone can help me with this? the aim is to reduce the size of output.