Best way to check posttranslation mod-s?

Question

[f.a.galkin]

I want to see whether the protein I work with has any phosphorylation or glycosylation sites and what enzymes regulate these potential modifications?

I hear AMS4.0 can do that for me, but I don't know how to use it.

[David Westergaard]

Have you tried simply looking up the protein at http://uniprot.org?

Answer 1

[Jean-Karim Heriche]

Phospho.elm BLAST allows you to check if your sequence contains any of their curated phosphopeptides. Another possibility is to use Networkin to predict phosphorylation sites.