RNA-Seq Introductory Material

Question

[NetunoPoncã]

Hello,

I used to work with microarray data. When I was beginning in the field I found several good and introductory reviews that helped a lot for someone with computer science background. For instance:

1. Alvis Brazma, Jaak Vilo, Gene expression data analysis, FEBS Letters, Volume 480, Issue 1, 25 August 2000, Pages 17-24
2. Christina A Harrington, Carsten Rosenow, Jacques Retief, Monitoring gene expression using DNA microarrays, Current Opinion in Microbiology
3. Adi L. Tarca, Roberto Romero, Sorin Draghici, Analysis of microarray experiments of gene expression profiling, American Journal of Obstetrics and Gynecology

Can anyone recommend any similar introductory material regarding RNA-Seq?

All the best!

Answer 1

[GouthamAtla]

You will get may if you search though google. There are plenty of tutorials and review papers available but you can start with NGS and then RNA-seq. Read few articles on RNA-Seq data analysis, like cuffdiff protocol (though many new tools have come, its still a good start). Once you understand the basic data analysis pipeline, start exploring the tools like HTSeq-count, edgeR/DESeq etc.

There are many threads in biostars as well. E.g: How Do I Get Started Working With Rna-Seq Data

Answer 2

[Charles Warden]

The following pages have information for both RNA-Seq and microarray analysis, but it may still be worth a quick look since I have tried to list the most popular programs used for various types of analysis:

• http://cdwscience.blogspot.com/2013/03/bioinformatics-101-rna-sequence-analysis.html
• http://cdwscience.blogspot.com/2013/03/bioinformatics-101-gene-expression.html