Entering edit mode
9.3 years ago
Biomonika (Noolean)
3.2k
I want to use Quiver as a standalone tool to polish my final assembly produced by different set of tools. What is recommended way to generate bam file that Quiver needs (maybe blasr, bwa mem)? Thanks.
quiver -j8 aligned_reads.bam \
-r path/to/lambda.fasta \
-o variants.gff -o consensus.fasta
I didn't have the opportunity to work with PacBio data yet, but from what I've read, you should use pbalign, which is a wrapper around several tools which will generate alignments for Quiver.