Entering edit mode
9.3 years ago
always_learning
★
1.1k
Dear All,
I have been using SNPSift for annotating VCF with database dbNSFPv_2.6.txt.gz and its working perfectly. Now I am using SNPSift with dbNSFP2.9.txt.gz version for few other annotation like ExAC frequency but getting Null Pointer exception.
If some one else got same error while trying dbNSFP2.9.txt.gz with SNPSift?
Here is command that I am using and associated errors too.
java -jar /gpfs/software/genomics/snpEff/4.1A/SnpSift.jar dbnsfp -v -db /gpfs/data_jrnas1/ref_data/Hsaens/GRCh37/variation/dbNSFP2.9.txt.gz GA_151.GATK.annotated_10000.vcf
00:00:00.000 SnpSift version SnpSift 4.1a (build 2015-01-14), by Pablo Cingolani
00:00:00.003 Command: 'DBNSFP'
00:00:00.003 Reading configuration file 'snpEff.config'
00:00:00.282 done
00:00:00.283 Annotating
Input file : 'GA_151.GATK.annotated_10000.vcf'
Database file : '/gpfs/data_jrnas1/ref_data/Hsapiens/GRCh37/variation/dbNSFP2.9.txt.gz'
Exception in thread "main" java.lang.RuntimeException: java.lang.NullPointerException
at ca.mcgill.mcb.pcingola.snpSift.SnpSiftCmdDbNsfp.annotate(SnpSiftCmdDbNsfp.java:100)
at ca.mcgill.mcb.pcingola.snpSift.SnpSiftCmdDbNsfp.run(SnpSiftCmdDbNsfp.java:423)
at ca.mcgill.mcb.pcingola.snpSift.SnpSiftCmdDbNsfp.run(SnpSiftCmdDbNsfp.java:408)
at ca.mcgill.mcb.pcingola.snpSift.SnpSift.run(SnpSift.java:360)
at ca.mcgill.mcb.pcingola.snpSift.SnpSift.main(SnpSift.java:69)
Caused by: java.lang.NullPointerException
at ca.mcgill.mcb.pcingola.snpSift.SnpSiftCmdDbNsfp.initAnnotate(SnpSiftCmdDbNsfp.java:350)
at ca.mcgill.mcb.pcingola.snpSift.SnpSiftCmdDbNsfp.annotate(SnpSiftCmdDbNsfp.java:98)