Using Sequin, cannot put in protein translation exception.

Question

[devenvyas]

Hello, I am attempting to use Sequin to prepare mitochondrial genome sequences for GenBank submission.

There are a handful of CDS's where the "TAA stop codon is completed by the addition of 3' A residues to the mRNA." I have left that note on all the relevant CDS's, but I am having issue inputting the translation exception to get the the "/transl_except=(pos:#..#,aa:TERM)" lines in my record viewer. I was wondering if someone could help me with this. What exactly do have I to put in the AA box to get this work? Thanks

Answer 1

[Hranjeev]

You've got to key in the amino acid number after setting a 3' coordinate on the Location tab (update your coordinate accordingly to consider the TA overhang). Your amino acid number will usually be total AA_length + 1. And, sequin will calculate the exception position for you.

Answer 2

[samh]

I would like to add the option "/transl_except=(pos:#..#,aa:TERM)" , but I couldn't find any info how to do it. thanks