Question

Recent RNA-Seq Analysis reviews

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9.3 years ago

wariobrega ▴ 60

Hi Everyone, first time posting here so I'm pretty excited!

I am pretty new to RNA-Seq (I studied it a little bit during my MSc, but very generally), and I am facing my first RNA-Seq analysis. I was searching for some recent review (last couple of years) discussing the pros and the cons of each RNA-Seq method, as well as a general little "tutorial" for RNA-Seq analysis, but I am not finding anything dated after 2011. Can you point me to one pf these papers and/or a general tutorial on RNA-Seq analysis, from QC to transcript reconstruction?

EDIT: sorry I didn't mention, the purpose of my RNA-Seq study is a classic Differential Expression Analysis of two conditions! Thanks a lot for your reply though!

Thanks a lot and have a nice day!

RNA-Seq • 1.8k views

ADD COMMENT • link updated 2.1 years ago by Ram 44k • written 9.3 years ago by wariobrega ▴ 60

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Could you be a little more specific. RNA-Seq data can be used for various king of analysis. Gene expression, Transcript expression, differential splicing events, fusion genes, ...

ADD REPLY • link 9.3 years ago by Nicolas Rosewick 11k

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I am sorry I didn't mention it, it's for classic DE analysis!

ADD REPLY • link 9.3 years ago by wariobrega ▴ 60

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Check out this amazing website: http://rnaseq.uoregon.edu/

ADD REPLY • link 9.3 years ago by Floris Brenk ★ 1.0k

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The RNA-seq blog has great news and papers about RNA-seq analysis.

ADD REPLY • link updated 2.1 years ago by Ram 44k • written 9.3 years ago by tiago211287 ★ 1.5k

score 4 · Answer 1 · 2015-08-19

Check Lior Pachter blog. It has several excellent posts on several issues related to RNAseq, and he maintains a *seq page with dozens (getting close to 100) references.

If you start by reading his latest post, you will find links to several other RNAseq posts.

edit: also the Simple Fools Guide here and here.