Hi All,
I'm trying to use different tools for detection of differential alternative splicing from RNAseq data, specifically MISO and rMATS. But both of them need RNAseq data with a defined read-length. Unfortunately, my data has a very wide range of read length from 50 to 185. I know that I should unify those length around the most occurrence read length which in my case is 140 bp reads in order to minimize information loss. So But I am not sure I know any tool that I can use to do that. I'd really appreciate if you know such tool to suggest them.
Thanks
Thanks for informing me about this tool, It sounds like a nice one.
I tried to use your command. But apparently it is output was almost trimmed like 52% of my input reads, which is like a huge percent of it has been trimmed
Is it a sign that I shouldn't filter based on read length?!
Do you have any suggestions?