How to normalize RNAseq raw read counts with out control?

Question

[ifudontmind_plzz]

Hi,

I have few RNAseq data (raw read counts) from mouse tumor samples. I don't have any controls or biological replicates. How can I normalize these type of data set?

[Matt Shirley]

You could median normalize every row across samples. That way you can still identify extreme changes.