Blast+ standalone : database creation , install BLAST+ locally

Question

[jinutchirayath]

Hello all,

I have installed NCBI-Blast-2.2.28 on win-7 32 bit system. Also set the path variable to bin directory. I want to create database of plant microRNAs. I have downloaded mature micoRNAs from miRBase. Right now I am having an excel file containing plant microRNas from miRBase

I want to convert this data in to blastable format. I have converted the excel file into csv format and then in to fasta format and tried makeblastdb command, but it outputs BLAST options error: file doesn't exist. Can you please help me regarding this?

Thanks for your help in advance

[thackl]

Can you post the commands you used to create the fasta and create the database. This sounds like a generic command line problem, something like a spelling error - not something actually related to blast.

[DG]

Yup, sounds like the path to the file isn't being specified correctly or a typo somewhere.

[h.mon]

Do not use spaces on any folder and file names involved. No spaces, ever.