Rnaseq calibration dataset

Question

[Raygozak]

HI, i know that there is the SRA for RNASeq datasets, but is there a dataset including scripts for which i could replicate the results and be certain that i'm not doing anything wrong in my rnaseq pipeline?

The description in most papers is too hand wavy and you need a lucky strike to reproduce it.

Thanks

Answer 1

[Ido Tamir]

There is the SEQC with uses human brain?/liver? RNA-seq standards, and there is a complete pipeline in the repository: https://bitbucket.org/soccin/seqc

paper: http://www.genomebiology.com/2013/14/9/R95

I think they used a subset of this (1 Tb of data): http://bioconductor.org/packages/release/data/experiment/html/seqc.html

Answer 2

[Raygozak]

Just for reference I happened to find another dataset from Drosophila RNASeq. This is a simulated one.

http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE32038