Trim barcode 3'

Question

[pingEde]

Hello,

I have an question: I have to trim barcode at 3' and the sequence is reversed, cutadapt is able to remove this sequence or I have to do also in the file barcode.fasta the reverse sequence?

Could you suggest a good tool to remove a list of primer in the read?

Thank you very much for your help

[glihm]

What do you mean by "reversed"?

5' - ACCGGGTTT - 3'

Which gaves: TTTGGGCCA,

or with complementary reversion TGGCCCAAA?

[pingEde]

Complementary reversion.

Thank you for your help!