Demultiplexing at 3'

Question

[pingEde]

Hello!

I have a problem with demultiplexing, I need a tool that it is able to check barcode at the end of the reads (3'). In particular my problem is that 200 reads are not assigned, so if I will check also at the 3' end I can assign this reads (because also in 3' end I have barcode in complementary reversion) . Is there a solution?

Thank you very much in advance

[Martombo]

200 reads over what total?

Answer 1

[pingEde]

17000, I know that 200 reads is a small number, but I would like know if there is a tool that checks at 3'