SRA to fastq
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9.2 years ago
aj123 ▴ 120

Is there some way to convert SRA to fastq? I'm on a windows system and the sra toolkit is not working on my system.

fastq next-gen-sequencing sra • 3.7k views
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You can try compiling the SRA toolkit under cygwin (didn't try, caveat emptor).

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dear all I am trying to convert SRR10756635, SRR10756640, SRR10756644 and SRR10756675 SRA files to fastaq but nothing is working for me at all. Can anyone help?

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Please open a new question and add sufficient details. "Does not work" is not an appropriate description, please add error messages or descriptions of what is (not) happening. You might simply doenload them via sra-explorer.info directly in fastq format.

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9.2 years ago
James Ashmore ★ 3.5k

You could upload your SRA files to Galaxy and use the SRA to FASTQ converter, however I'd strongly recommend you try to get the SRA toolkit working on your Windows machine.

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6.4 years ago
ATpoint 85k

There is now a new release of the SRAtoolkit (2.9.1) that introduces fasterq-dump, a multi-threaded follow-up of fastq-dump. Had a quick look at it and the increase in speed is notable, but (and I really do not understand this in 2018 at all) there is no longer a --gzip option, requiring additional post-processing. I really do not understand why, in times where projects may require the processing of terabytes of .sra data, a tool can lack a compression option, flooding the hard drives with Tb of unnecessarily large amounts of data (the last sentence is of course thinking aloud). I will probably stick with parallel-fastq-dump, given that files (e.g. from dbGaP) are not backed up on ENA.

Also see [Deprecated] Fast download of FASTQ files from the European Nucleotide Archive (ENA)

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9.2 years ago

At first check your system specification. Download SRA toolkits accordingly(32 bits/ 64 bits)

That might solve the problem

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6.4 years ago

Not quite an answer to the question you are asking, but the ENA (European Nucleotide Archive) has all publically available SRA samples for download directly as fastq. The download will take much longer (unless you use aspera), but the you more than make up for it in conversion time.

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And ENA makes it easier to retrieve the metadata as well, for both human and machine consumption.

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7.6 years ago

From SRA toolkit => "./fastq-dump SRA* "

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