Blast2Go .annot file to tree

Question

[Joefromlondon]

Hi Guys,

I have been given a .annot file from Blast2go by my superviser, and I am trying to create a full top down tree using this. It only creates the end nodes, and wondered if there was an easy way to generate the data going from the nodes back to the root of the tree.

Thanks in advance, and feel free to ask for more details.

J

Answer 1

[Stefan]

I guess by "a full top down tree" you mean a Gene Ontology Direct Acyclic Graph (DAG).

You can do this with Blast2GO in various manners. If you already have the .annot file you can simply load this file and click on "Combined Graph" which will draw a full GO DAG of your functional annotations.

If by "it created only the end nodes" you mean Blast2GO shows only the leaf nodes please set your drawing parameters back to default (click the default button in the wizard) and take a look at the "Sequence Filter" to make sure you do not filter out or trim any GO nodes.

Another option for more advanced GO DAG visualization is the "Coloured Graph". You can provide a list of GOs with additional optional group and numeric attributes to view GOs with discrete colours for different groups (e.g. experiments) or with gradual color changes to represent for example p-values associated to your functional information.

Hope this answers your question.