Entering edit mode
9.2 years ago
evobio
•
0
SCENARIO example:
- Go to Ensembl Zv9 (http://mar2015.archive.ensembl.org/Danio_rerio/Info/Index).
- Search for gene "ghra".
- Go to the "Location" tab.
- Click on "Configure this page" on the menu on the left.
- Click on "RNASeq models", under "Genes and transcripts".
- Select all the rows on the "Exons" column.
- Save and close (check mark on the upper left).
Now we can see the developmental stages at which the transcripts related to that gene are expressed, according to RNA-seq data.
QUESTION:
Is it possible to download the list of all transcripts and genes that are expressed at a specific developmental stage in zebrafish? If yes, how? I'm new in bioinformatics. I have tried BioMart, but it doesn't have the option for RNASeq per developmental stage.
I also wasn't able to find the appropriate track in BioMart (or biomaRt), but that doesn't mean it's not there. I did find this reference:
http://genome.cshlp.org/content/22/10/2067.long
Which describes connections to the otherfeatures database with Perl. I think the answer is in there, but you would need to learn at least a little Perl to get the syntax right. Good luck!