The first amplicon librariesis is needed to analyse the 16S data?

Question

[m.koohi.m]

Hi,

For analyzing 16S data with Mothur or Qiime is it necessary that I have the the first amplicon libraries sequences or not? The data is produced by MiSeq illumina multiplexing technology and the barcode of each sample is provided by the lab.

Best

Answer 1

[glihm]

Hi,

if you are looking for OTUs from sequencing data in order to compare microbial communities in your samples, you will only need FastQ files from sequencing.