using DEseq to produce RPKM and VST

Question

[zizigolu]

Good morning friends,

At last I produced a file containing the reads raw counts. Now I should prepare VST and RPKM by DEseq, but I am not familiar with R. Only rstudio and DEseq package installed. Do you know any script or a way to start?

Thank you

[Anton Shekhov]

Is not this what you are looking for? A: Calculating RPKM for DESeq

If yes, than here is good work-through solution https://github.com/mgonzalezporta/TeachingMaterial/blob/master/doc/25.normalising.md

[Nicolas Rosewick]

Read DESeq vignette. It's well written and easily understandable