How to figure out coding sequence based on the results of blastx

Question

[grayapply2009]

Hey guys,

I have annotated my sequences with Blast2go. Now I need to label the coding sequence (find out start and stop codon). How do I do it? Does Blast2go have this function?

By the way, after annotation, I've got the full gene names and gi numbers for each sequence. How do I find gene symbols for each sequence?

Answer 1

[Prakki Rama]

Try running Augustus or ORF finder

Answer 2

[vahapel]

If you did your analysis in de novo trascriptome assembly manner, I recommend "TransDecoder Tool" (https://transdecoder.github.io/), it is good in terms of quality and fastness.