How to use WEGO (Web Gene Ontology Annotation Plot)

Question

[amyfm]

Hi,

I have an Excel file with around 1000 genes of interest to me and I want to do pathway analysis. I read about WEGO, an online tool to get nice graphics of the pathways, but I don't know how to use it. I don't know which file I should upload.

Previously, I go to gene ontology website, and I use Panther classification system to get information about cellular process, molecular functions, etc. I download some output files and upload them to WEGO but it is not working.

Can anybody explain to me how to use WEGO starting from an excel file with genes?

Thank you

Answer 1

[Guangchuang Yu]

wego stop updating their data since 2009. see http://ygc.name/2015/02/01/kegg-enrichment-analysis-with-latest-online-data-using-clusterprofiler/

I recommend you not to use it.

Answer 2

[lakhujanivijay]

This is an update that I wanted to share. It seems that the wego wesite got a makeover recently and appears quite better

Just have a look http://wego.genomics.org.cn/

Though I am still struggling to get a good quality bar plot in png format.