Converting tags to gene names in GPL9115
1
0
Entering edit mode
9.0 years ago
Na Sed ▴ 310

I have downloaded GSE31617 data. As you can see its platform is GPL9115.

In mRNA files, there are three columns:

       Tag                            CopyNumber    TPM(Transcript Per Million)
CATGTGGATGGGCTTCTTGTA                    2                          0.23
CATGGGGCCTTCCAGACCCAC                    2                          0.23
CATGTGCATTTTCAAGTGGGT                    2                          0.23
CATGAGCCACCGCGCCCTGTC                    2                          0.23
CATGGAACTAATTCGCTGACC                    2                          0.23
CATGCTGCTTCGGCCCCAGCG                    2                          0.23
CATGGTCCTCACCCAAGCCTA                    2                          0.23
CATGTCTGTGTGTGGTGAGCA                    2                          0.23

How can I map Tag to RefSeq ID or gene symbol? My goal is making a matrix of expression profile.

Unfortunately, I did not find any useful information in GPL9115. SOFT formatted family file(s) in this page contains something like above columns for all of the available samples in GEO.

I appreciated it if anyone could help me.

RNA-Seq ChIP-Seq GPL9115 RefSeq-ID • 1.9k views
ADD COMMENT
2
Entering edit mode
9.0 years ago
Michael 55k

You did not read http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM785379 ?

You should align the short tags to the genome using a (very) short read aligner, then overlap the alignments with the genome annotation as in a standard RNA-seq pipeline.

ADD COMMENT
0
Entering edit mode

Thanks for your help.

ADD REPLY

Login before adding your answer.

Traffic: 1832 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6