error in command

Question

[Bulbul Ahmed]

/trinityrnaseq/util/align_and_estimate_abundance.pl \
    --transcripts reference.fasta \
    --seqType fq \
    --left /home/file/read1.fastq \
    --right /home/file/read2.fastq \
    --est_method RSEM \
    --aln_method bowtie \
    --trinity_mode

above command i used but i found error given below (lines folded for readability)

rsem-calculate-expression --paired-end -p 4 
    --no-bam-output --bam bowtie.bam 
    /home/reference.fasta.RSEM 
    RSEM died with ret: 65280 at /trinityrnaseq/util/align_and_estimate_abundance.pl line 632

any body can find the what actually it needs?

[iraun]

Maybe its a silly comment but, are you sure that your left and right files have read1.fastq and data2.fastq names, respectively?

[Bulbul Ahmed]

sory its read1.fastq and read2.fastq

[Ram]

Looks like something is missing at the end - errors usually include a line number after the "xyz.pl line" part

[allysonm.weir]

Hi, I know this was posted almost two years ago, but I have the exact same input and error! Were you ever able to figure it out?