Can you use Next Generation Sequencing (NGS) establish if a gene is pathogenic

Question

[adewale]

Having Identified a nucleotide change in a gene, how would we establish if it's pathogenic?

[Istvan Albert]

You need to understand what effect the mutation has and it how it affects the protein that it translates to. If you are asking for a a simple tool or straightforward or generic method ... there probably isn't one.

[apelin20]

No (as others said). NGS tells you sequence, not function.

Answer 1

[swbarnes2]

You would need empirical evidence that the mutation damages gene function so badly it causes a deleterious health phenotype. No computer can do that for you.

Answer 2

[jotan]

Making the mutation in a model organism is the only way to prove this.