what the "cycle" mean in MeanQualityByCycle of picard

Question

[zengxi.hada]

Hi,

I use picard tool "CollectBaseDistributionByCycle" to plot a figure "CollectBaseDistributionByCycle.pdf". But I have no idea about what's the mean of cycle of x lab. Who can tell me what it means? Thanks.

Best,
Michael

Answer 1

[Devon Ryan]

A cycle is a base. To make a 100 base read, the sequencer cycles through a given procedure 100 times, which is where the term comes from.

[zengxi.hada]

thank you. quite clear.