For transposon mapping, how important is sequence length? Is Illumina 75x2 PE sufficient versus the 150x2 expense?
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9.0 years ago
Daniel ★ 4.0k

We have a couple of 75x2 kits available for our new NextSeq and we're looking at a transposon analysis (not something I've looked into before).

Is this sufficient for such an analysis? I would imagine that longer read lengths would provide better coverage of the intersections and thus be better suited, but are there diminishing rewards, and the extra expense is not worthwhile?

Thanks

NGS transposons • 1.6k views
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