Tool:Don't Clone Alone
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9.0 years ago
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The New Restriction Ligation Cloning Wizard by Genome Compiler

Genome Compiler, an all-in-one DNA design software platform (which is free for academics), has a great cloning wizard to make your life easier by simplifying everyday molecular biology assignments. This wizard was released a few months ago, and after receiving great feedback and some requests from it's users, a whole new Restriction Ligation Cloning wizard was developed and got a whole lot better, easier and smarter.

The new cloning wizard has a whole new user interface to further simplify and guide the design of your cloning experiments.

Using the wizard, you can simulate digest and ligate with restriction enzymes and also simulate digest and ligate with PCR. Learn how to use it by watching the tutorial videos.

The New Restriction Ligation Cloning Wizard

What's the new Cloning Wizard all about?

  1. Time Saver. Auto-detect design errors with the new predictive design engine.
  2. Flexible. Seamlessly add modifications to restriction sites on your insert or backbone with 5' and 3' overhangs.
  3. Simple. Choose the exact fragment when using multi-cutter restriction enzymes.
  4. Versatile. Generate your backbone and insert using endogenous restriction enzymes, PCR or synthesis.
  5. Intuitive. Easily visualise and verify your backbone and insert sticky ends.
  6. Useful. For PCR, design your own primers, auto-design them with Primer3 or choose primers directly from your primer libraries
RNA-Seq Assembly gene • 1.9k views
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