Running BLAST+(version 2.2.31) locally for GRCH37 refseq
0
0
Entering edit mode
9.0 years ago
niharraul • 0

I am trying to use BLAST updateblastdb.pl refseq_genomic script to download human genomic datasets so that I can run the BLAST software locally. However, I realized these datasets would be for GRCh38 (the latest versions) and not GRCh37.

  1. My question is to how to get the files for GRCh37?
  2. What command should I be using to run it locally. Since I will be aligning a chunk of different DNA sequences(query sequences could be up to 5000 in number) of various length from 20 bp to say 120 bp? Since if using protein database blastp is used, blast for nucleotide database and etc.
genome blast alignment • 2.2k views
ADD COMMENT

Login before adding your answer.

Traffic: 2880 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6