Entering edit mode
12.8 years ago
Nicolas Rosewick
11k
Hi,
Which type of preprocessing do I have to performe on my reads before doing a de-novo transcriptome assembly (Trinity) ? My data comes from a Illumina GAII plateform (paired-end 2x72 bp). Do I have to trim the 3' adapter sequence ?
Thanks a lot,
N.
This is correct, your first step would be removal of the 3' adapters, followed by quality trimming. This will also mostly trim something from the 3' ends of the read. You probably should also do
fastqc
as a norm so that you know your data in general is in good standing.