Identifying distance between mate pairs
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9.0 years ago

I would like to know of a few tools for identifying distances between mate pairs on datasets where that is not given.

RNA-Seq matepairs tophat trinity • 2.5k views
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9.0 years ago
h.mon 35k

You can get a biased estimate by merging overlapping pairs and measuring the resulting sequence sizes, or you can map to a reference genome and estimate insert size. BBMerge can merge reads and output an insert size histogram, BBMap can map and also output an insert size histogram.

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