Question

Tools to compute Lorenz curve of coverage uniformity

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9.0 years ago

igor.fobia ▴ 10

I would like to compute a Lorenz curve of per-base coverage depth for a BAM file on a certain BED file. I can find a way to do it by myself, but it would be great if you could point me to some existing software.

Thank you in advance!

bam genome alignment • 3.0k views

ADD COMMENT • link updated 18 months ago by Ram 44k • written 9.0 years ago by igor.fobia ▴ 10

score 2 · Answer 1 · 2021-12-23

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3.0 years ago

yhoogstrate ▴ 150

Hi Igor,

I wrote a python tool to do so:

https://github.com/yhoogstrate/bam-lorenz-coverage

all best,

Youri

ADD COMMENT • link 3.0 years ago by yhoogstrate ▴ 150

Ram · Answer 2 · 2016-01-05

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9.0 years ago

igor.fobia ▴ 10

htSeqTools (with giniCoverage function) seems the only ready-to-use tool I have found for this task. However, it does not allow to restrict the analysis on a specific region (for example, by using a BED file).

ADD COMMENT • link updated 2.4 years ago by Ram 44k • written 9.0 years ago by igor.fobia ▴ 10

score 0 · Answer 3 · 2021-12-23

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3.0 years ago

biology_inform ▴ 50

https://blog.addgene.org/ngs-quality-control-for-pooled-libraries you can check the comments there is a comment by will including a link which is plsa.py

ADD COMMENT • link 3.0 years ago by biology_inform ▴ 50