Is there a way to add xs tag to a sam file?

Question

[dina.hesham139]

Hi,

I did the alignment to my fastq files using STAR. However, I didn't include --outSAMstrandField intronMotif option in the command. So, the resulting sam files are lacking xs tags which are necessary for downstream analysis (Stringtie/cufflinks). Is there a way to add xs tag to a sam file after the alignment, or I have to redo the alignment?

Thanks

Answer 1

[Devon Ryan]

Just redo the alignment.