Question

single end miRNA analysis

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8.8 years ago

anithanagaraj93 ▴ 10

Hi,

Any one tell why we use only single end fastq files for miRNA analysis not paired end? what is the reason?

gene • 1.4k views

ADD COMMENT • link updated 2.3 years ago by Ram 44k • written 8.8 years ago by anithanagaraj93 ▴ 10

Ram · Answer 1 · 2016-01-25

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8.8 years ago

Devon Ryan 104k

miRNAs are shorter than a typical single-end read, so read #2 would just be wasted bases (it'd completely overlap read #1).

ADD COMMENT • link 8.8 years ago by Devon Ryan 104k

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Thanks Devon... I have one more doubt what is 3p and 5p actually in miRNA result nomenclature?

ADD REPLY • link updated 4.9 years ago by Ram 44k • written 8.8 years ago by anithanagaraj93 ▴ 10

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"Question", not "doubt".

Anyway, see the wikipedia article here.

ADD REPLY • link updated 4.9 years ago by Ram 44k • written 8.8 years ago by Devon Ryan 104k

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Thank Devon for your patience...

ADD REPLY • link 8.8 years ago by anithanagaraj93 ▴ 10