Hi,
I am interested in structural changes in my protein due to mutation(s). I have WT pdb structure and I would like to model structure after single or multiple mutations . I would like to use something more fancy than just choosing from rotamer library. I was thinking about RosettaBackrub or Modeller or maybe there is another tool?
Thx for any suggestions!
thanks I will give a try
Be careful not to introduce too many mutations. The structure can be very unpredictable. In protein switches it takes just one mutation to switch the structure completely. You might try TINKER (http://dasher.wustl.edu/ffe/) as well. I think it'll do we with just "wiggling" the new sidechain into its best position.
Be careful not to introduce too many mutations. The structure can be very unpredictable. In protein switches it takes just one mutation to switch the structure completely. You might try TINKER dasher.wustl.edu/ffe as well. I think it'll do well with just "wiggling" the new sidechain into its best position.