gene expression candidate for RT-PCR

Question

[loly.pearl86]

Hi All,

I want examine different gene expression for different treatment by using qRT-PCR, I will not do any sequencing or assembly. First RNA will be extract and then my question is How can I choose the candidate genes before run PCR? Then I can design the primer for the candidate and run PCR.

I hope if someone can explain and suggest methods for chose the candidate.

Thanks

Answer 1

[karl.stamm]

Every gene is a candidate until you can specify what question you want to ask of which animal and which tissue. In a muscle, a muscly-gene is important, and in glands some metabolic genes are interesting.

Your question is like a child learning to add and subtract and asking which numbers to try it on. All the numbers are good.