How does MITObim (or MIRA's mapping mode) call bases in the consensus?
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8.8 years ago
novice ★ 1.1k

I've been reading the MITObim paper trying to find out how exactly does it call bases in the final consensus. It doesn't seem to include this information and nor does the MIRA paper. Maybe because it's expected to be known?

Here's an example of what I'm asking.

   AATCG?TTTCATG      Final contig
     TCGATTT          Reads
     TCGCTTT
     TCGCTTT
     TCGCTTT

In a situation like this, would the base in the final contig be C? Is it determined simply by majority? Is this how most consensus assemblers work?

MITObim MIRA mapping • 2.1k views
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