Normalization of CLIP peak by transcript level
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8.8 years ago

HI,

I am using Piranha for CLIP-Seq data analysis. I was wondering how to normalize peaks by transcript abundance (calculated as FPKM from RNA-seq). I read in several articles that normalization with transcript level could help remove spurious peaks. I therefore performed RNA-seq as well, together with CLIP-Seq for the corresponding samples. Could some body please help me out. Any response is highly appreciated.

RNA-Seq • 2.1k views
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