Entering edit mode
9.2 years ago
super
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60
Hi All
I have a simple question. When I ran DESeq2, I got the error.
sampleclass<- data.frame(condition=factor(c(rep("C1",3),rep("C2",3))))
dds<-DESeqDataSetFromMatrix(countData=counts, colData= sampleclass, design=~condition)
Error in SummarizedExperiment(assays = SimpleList(counts = countData),
The SummarizedExperiment class defined in the GenomicRanges package is
deprecated and being replaced with the RangedSummarizedExperiment class
defined in the new SummarizedExperiment package. Please make sure to
install the SummarizedExperiment package before you attempt to call the
SummarizedExperiment() constructor function. Note that this will return
a RangedSummarizedExperiment instance instead of a SummarizedExperiment
instance.
> head(counts)
C1.R1 C1.R2 C1.R3 C2.R1 C2.R2 C2.R3
ENSRNOG00000000001 516 498 451 328 389 424
ENSRNOG00000000007 5 4 4 6 1 1
ENSRNOG00000000008 9 2 7 3 4 4
ENSRNOG00000000009 1 2 6 0 1 2
ENSRNOG00000000010 1 0 3 0 0 0
ENSRNOG00000000012 1 0 2 0 0 0
> head(sampleclass)
condition
1 C1
2 C1
3 C1
4 C2
5 C2
6 C2
Why? I have installed the latest version of SummarizedExperiment().
Make sure you have the most recent version of everything. It's likely that this was addressed in the most recent version of DESeq2.