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9.1 years ago
kanwarjag
★
1.2k
I have RNAseq data as RPKM values (tumor vs normal) since data is paired end how could I convert to FPKM values.
Thanks
I have RNAseq data as RPKM values (tumor vs normal) since data is paired end how could I convert to FPKM values.
Thanks
In case of single end data, RPKM=FPKM (R
eads p
er k
ilobase per m
illion reads and F
ragments p
er k
ilobase per m
illion reads).
In case of paired end data, you have for every read-pair one fragment. Thus, divide the RPKM by two.
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You
may/should not convert RPKM to FPKM
. You need to recalculate FPKM from BAM file. As suggested below, if you just divide by 2, they wont be true FPKM values as you do not knowif there are any read-pairs with only one-end mapped.
And if there are many read pairs like that your FPKM value might not be true.Thanks for the explanation