Entering edit mode
8.7 years ago
jnf3769
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40
I was wondering if anyone knew of a method by which to generate electropherograms or abi/abi files for a lab I am teaching. The only reason why I am looking for a slightly more realistic way is because the lab is behind schedule due to some issues with the sequencer. I want the students to use software to interpret the abi, align some sequences, and design their own internal primers for use later in the lab. I have the true sequence as a series of ABI/AB1 files already, but I'd like to introduce some errors/variation in the electropherogram.
Anyone have any clues or ideas? I am pretty familiar with R/Bioconductor, Java, and python, if that helps.
Thank you for your time!
