NCBI error report

Question

[archana.bioinfo87]

Hi all, I am getting this Message ID#29 Error: Query string not found in the CGI context; when I am trying to align two or more seq in BLASTP.

Kindly answer.

[Chris Miller]

You're going to have to provide more information. Is this via the NCBI website? What's are the sequences you're trying to blast? What settings are you using?

[archana.bioinfo87]

Yes, I am trying it from NCBI website with default settings. Thanks for reply

[archana.bioinfo87]

Thanks................................ :)

Answer 1

[gangireddy]

if you are not using standalone.

try NCBI COBALT which is meant for multiple sequence alignment.

http://www.ncbi.nlm.nih.gov/tools/cobalt/cobalt.cgi?LINK_LOC=BlastHomeLink

or you can use culstal omega of EMBL-EBI

http://www.ebi.ac.uk/Tools/msa/clustalo/

Answer 2

[Prakki Rama]

I ran into this error today, and I found out the reason for the error.

To resolve:

Just untick the Align two or more sequences box.

My blast search then worked like a charm!!