TargetP Error: zero output generated

Question

[Louis Kok]

Hi All, when I execute TargetP using the test data provided in the tool, I receive the following error:

./targetp -P test/one.fsa

error as below:

open: can't stat file
apparent state: unit 3 named HOWIN_in
lately reading sequential formatted external IO

The output is as below:

### targetp v1.1 prediction results #####################
Number of query sequences: 1
Cleavage site predictions not incuded.
Using PLANT networks.
Name          Len     cTP    mTP    SP     other    Loc     RC
------------------------------------------------------------------
P48786;               1088         0.000       0.000       0.000      0.000           ?             5
------------------------------------------------------------------
cutoff                                   0.000       0.000       0.000      0.000

I wonder if anyone of you has the same issue. Do let me know if there is any solution. Thanks in advance.

[emilygiroux]

I've been having the exact same issue. I also have the same issue with the standalone package for signalp-3.0: open: can't stat file apparent state: unit 3 named input.how lately reading sequential formatted external IO error running HOW

These packages are both obtained from the same source, but I can't seem to find much help for these packages online.

[Louis Kok]

I successfully run it in Ubuntu guest OS in my Windows 7 laptop even thought I still could not find the actual reason.

[Peter]

I've also had much the same error (plus the string error running HOW) from signalp-3.0 (from the same team) when running on our cluster. I believe this is a bug in the wrapper script for targetp and signalp which naively uses a predictable temporary folder, which leads to rival copies of the HOW binary clashing over access to the same temp file.

[cat.lou]

Did anyone manage to resolve this issue? I successfully installed and ran the test data for Signal P. However, I am getting the same error as detailed in the original question when I run the test data for Target P. TIA.