Hi Can we combine or correlate the results of NGS and Microarray data? In the results of GEO2R some of the value are missing? How i treat that probe id?
Hi Can we combine or correlate the results of NGS and Microarray data? In the results of GEO2R some of the value are missing? How i treat that probe id?
You should rename your question and/or ask two separate questions.
I've found that it is possible to compare microarray and RNA-Seq gene expression values by comparing the expression of one set of genes relative to another set of genes (in the links below, TCGA is RNA-Seq while all other datasets are microarrays).
https://peerj.com/articles/159/#table-3 https://peerj.com/articles/159/#fig-3
In general, the absolute values won't be comparable (and I wouldn't recommend doing anything like quantile normalization to make them similar), and the fold-change values for individual genes will probably also be different (although you could do independent analysis and look for overlapping genes).
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You can use missing value imputation. impute package form bioconductor