I have given the command 1-library(affy) #this returns Loading required package: BiocGenerics Loading required package: parallel
Attaching package: ‘BiocGenerics’
The following objects are masked from ‘package:parallel’: clusterApply, clusterApplyLB, clusterCall, clusterEvalQ, clusterExport, clusterMap, parApply, parCapply, parLapply, parLapplyLB, parRapply, parSapply, parSapplyLB
The following objects are masked from ‘package:stats’:
IQR, mad, xtabs
The following objects are masked from ‘package:base’:
anyDuplicated, append, as.data.frame, as.vector, cbind,
colnames, do.call, duplicated, eval, evalq, Filter, Find,
get, grep, grepl, intersect, is.unsorted, lapply, lengths,
Map, mapply, match, mget, order, paste, pmax, pmax.int,
pmin, pmin.int, Position, rank, rbind, Reduce, rownames,
sapply, setdiff, sort, table, tapply, union, unique,
unlist, unsplit
Loading required package: Biobase Welcome to Bioconductor
Vignettes contain introductory material; view with
'browseVignettes()'. To cite Bioconductor, see
'citation("Biobase")', and for packages
'citation("pkgname")'
raw.data=ReadAffy(verbose=TRUE) #this returns 1 reading C:/Users/admin/Documents/cels.CEL ...instantiating an AffyBatch (intensity a 287296x18 matrix)...done. Reading in : C:/Users/admin/Documents/cels.CEL Reading in : C:/Users/admin/Documents/GSM533286.CEL Reading in : C:/Users/admin/Documents/GSM533287.CEL Reading in : C:/Users/admin/Documents/GSM533288.CEL Reading in : C:/Users/admin/Documents/GSM533289.CEL Reading in : C:/Users/admin/Documents/GSM533290.CEL Reading in : C:/Users/admin/Documents/GSM533291.CEL Reading in : C:/Users/admin/Documents/GSM533292.CEL Reading in : C:/Users/admin/Documents/GSM533293.CEL Reading in : C:/Users/admin/Documents/GSM533294.CEL Reading in : C:/Users/admin/Documents/GSM533295.CEL Reading in : C:/Users/admin/Documents/GSM533296.CEL Reading in : C:/Users/admin/Documents/GSM533297.CEL Reading in : C:/Users/admin/Documents/GSM533298.CEL Reading in : C:/Users/admin/Documents/GSM533299.CEL Reading in : C:/Users/admin/Documents/GSM533300.CEL Reading in : C:/Users/admin/Documents/GSM533301.CEL Reading in : C:/Users/admin/Documents/GSM533302.CEL
3-data.rma.norm=rma(raw.data) #this returns
installing the source package ‘hu6800cdf’
trying URL 'https://bioconductor.org/packages/3.2/data/annotation/src/contrib/hu6800cdf_2.18.0.tar.gz'
Content type 'application/x-gzip' length 431388 bytes (421 KB)
downloaded 421 KB
* installing *source* package 'hu6800cdf' ... ** R ** data ** preparing package for lazy loading Warning: replacing previous import 'AnnotationDbi::head' by 'utils::head' when loading 'hu6800cdf' Warning: replacing previous import 'AnnotationDbi::tail' by 'utils::tail' when loading 'hu6800cdf' ** help
*** installing help indices ** building package indices ** testing if installed package can be loaded Warning: replacing previous import 'AnnotationDbi::head' by 'utils::head' when loading 'hu6800cdf' Warning: replacing previous import 'AnnotationDbi::tail' by 'utils::tail' when loading 'hu6800cdf' * DONE (hu6800cdf)
The downloaded source packages are in
‘C:\Users\admin\AppData\Local\Temp\RtmpU1iKzP\downloaded_packages’
Background correcting
Normalizing
Calculating Expression
Warning messages:
1: closing unused connection 3 (http://www.bioconductor.org/main.html)
2: replacing previous import ‘AnnotationDbi::head’ by ‘utils::head’ when loading ‘hu6800cdf’
3: replacing previous import ‘AnnotationDbi::tail’ by ‘utils::tail’ when loading ‘hu6800cdf’
Am I getting something relevant? Am I giving correct commands in correct orders? Please do help.