SNP effect on protein stability

Question

[kartikayprasad]

Hello,

i am doing an exercise in which i have to find the effect of the SNP`s on the protein stability. i have the sequenced genome and i know the position of the SNPs. is there any tool in which i just give the position of the snp and the changed nucleotide and it will tell me whether it is affect the protein stability or not.

i tried to use I-Mutant 2 but i have to give one by one protein seq and the snp position , and i have large data to analyse.

thanks in advance for your help

Answer 1

[Pol]

I use: http://cadd.gs.washington.edu/score

Selecting "Include underlying annotation in output (not only the scores)". You can obtain scores for different parameters.

You can upload a VCF file to analyze multiple positions. A VCF from NGS is not necessary, only the structure is necessary.